Multi-walled carbon nanotube length as a critical determinant of bioreactivity with primary human pulmonary alveolar cells

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dc.contributor.author Sweeney, Sinbad
dc.contributor.author Berhanu, Deborah
dc.contributor.author Misra, Superb K.
dc.contributor.author Thorley, Andrew J.
dc.contributor.author Valsami-Jones, Eugenia
dc.contributor.author Tetley, Teresa D.
dc.date.accessioned 2014-08-04T13:57:00Z
dc.date.available 2014-08-04T13:57:00Z
dc.date.issued 2014-11
dc.identifier.citation Sweeney, Sinbad; Berhanu, Deborah; Misra, Superb K.; Thorley, Andrew J.; Valsami-Jones, Eugenia and Tetley, Teresa D., “Multi-walled carbon nanotube length as a critical determinant of bioreactivity with primary human pulmonary alveolar cells”, Carbon, DOI: 10.1016/j.carbon.2014.06.033, Nov. 2014. en_US
dc.identifier.issn 0008-6223
dc.identifier.uri http://dx.doi.org/10.1016/j.carbon.2014.06.033
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/1367
dc.description.abstract Multiwalled carbon nanotube (MWCNT) length is suggested to critically determine their pulmonary toxicity. This stems from in vitro and in vivo rodent studies and in vitro human studies using cell lines (typically cancerous). There is little data using primary human lung cells. We addressed this knowledge gap, using highly relevant, primary human alveolar cell models exposed to precisely synthesised and thoroughly characterised MWCNTs. In this work, transformed human alveolar type-I-like epithelial cells (TT1), primary human alveolar type-II epithelial cells (ATII) and alveolar macrophages (AM) were treated with increasing concentrations of MWCNTs before measuring cytotoxicity, inflammatory mediator release and MAP kinase signalling. Strikingly, we observed that short MWCNTs (∼0.6 μm in length) induced significantly greater responses from the epithelial cells, whilst AM were particularly susceptible to long MWCNTs (∼20 μm). These differences in the pattern of mediator release were associated with alternative profiles of JNK, p38 and ERK1/2 MAP kinase signal transduction within each cell type. This study, using highly relevant target human alveolar cells and well defined and characterised MWCNTs, shows marked cellular responses to the MWCNTs that vary according to the target cell type, as well as the aspect ratio of the MWCNT. en_US
dc.description.statementofresponsibility by Sinbad Sweeney, Deborah Berhanu, Superb K. Misra, Andrew J. Thorley, E. Valsami-Jones and Teresa D. Tetley
dc.format.extent vol. 78, pp. 26-37
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Carbon en_US
dc.subject Bioreactivity en_US
dc.subject Multiwalled carbon nanotube (MWCNT) en_US
dc.subject Macrophages en_US
dc.title Multi-walled carbon nanotube length as a critical determinant of bioreactivity with primary human pulmonary alveolar cells en_US
dc.type Article en_US
dc.relation.journal Carbon


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