Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles

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dc.contributor.author Misra, Superb K.
dc.date.accessioned 2016-12-17T08:43:00Z
dc.date.available 2016-12-17T08:43:00Z
dc.date.issued 2017-06
dc.identifier.citation Misra, Superb K. et al., “Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles”, Archives of Toxicology, DOI: 10.1007/s00204-016-1897-2, Jun. 2017. en_US
dc.identifier.issn 0340-5761
dc.identifier.issn 1432-0738
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/2561
dc.identifier.uri http://dx.doi.org/10.1007/s00204-016-1897-2
dc.description.abstract The rapid development of nanotechnologies and increased production and use of nanomaterials raise concerns about their potential toxic effects for human health and environment. To evaluate the biological effects of nanomaterials, a set of reliable and reproducible methods and development of standard operating procedures (SOPs) is required. In the framework of the European FP7 NanoValid project, three different cell viability assays (MTS, ATP content, and caspase-3/7 activity) with different readouts (absorbance, luminescence and fluorescence) and two immune assays (ELISA of pro-inflammatory cytokines IL1-β and TNF-α) were evaluated by inter-laboratory comparison. The aim was to determine the suitability and reliability of these assays for nanosafety assessment. Studies on silver and copper oxide nanoparticles (NPs) were performed, and SOPs for particle handling, cell culture, and in vitro assays were established or adapted. These SOPs give precise descriptions of assay procedures, cell culture/seeding conditions, NPs/positive control preparation and dilutions, experimental well plate preparation, and evaluation of NPs interference. The following conclusions can be highlighted from the pan-European inter-laboratory studies: Testing of NPs interference with the toxicity assays should always be conducted. Interference tests should be designed as close as possible to the cell exposure conditions. ATP and MTS assays gave consistent toxicity results with low inter-laboratory variability using Ag and CuO NPs and different cell lines and therefore, could be recommended for further validation and standardization. High inter-laboratory variability was observed for Caspase 3/7 assay and ELISA for IL1-β and TNF-α measurements. en_US
dc.description.statementofresponsibility by Superb K. Misra et al.
dc.format.extent Vol. 91, no. 6, pp. 2315–2330
dc.language.iso en_US en_US
dc.publisher Springer Verlag en_US
dc.subject Inter-laboratory studies en_US
dc.subject Standard operating procedures en_US
dc.subject Interference en_US
dc.subject In vitro assays en_US
dc.subject Nanoparticles en_US
dc.title Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles en_US
dc.type Article en_US
dc.relation.journal Archives of Toxicology


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