Dimeric carbocyanine dye and nucleic acid aptamer mediated detection of food borne toxin

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dc.contributor.author Chilka, Pallavi
dc.contributor.author Pallavi, Patlolla
dc.contributor.author Reddy, Prathap
dc.contributor.author Datta, Bhaskar
dc.date.accessioned 2018-02-28T10:05:08Z
dc.date.available 2018-02-28T10:05:08Z
dc.date.issued 2018-02
dc.identifier.citation Chilka, Pallavi; Patlolla, Prathap Reddy and Datta, Bhaskar, “Dimeric carbocyanine dye and nucleic acid aptamer mediated detection of food borne toxin”, Indian Journal of Chemistry -Section B, vol. 57B, pp. 281-286, Feb. 2018. en_US
dc.identifier.issn 0975-0983
dc.identifier.issn 0376-4699
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/3487
dc.description.abstract Food borne toxins represent a serious challenge to the advances in the operation of the food industry with respect to food processing and distribution. While selective identification of the toxins can be easily accomplished using standard chromatographic and mass spectrometric methods, these are not conducive for use in the field. In this work, we use nucleic acid aptamers in a label-free strategy for detection of specific food borne toxins. Our approach relies on the non-covalent association of a dimeric carbocyanine dye with the quadruplex secondary structures formed by aptamers specific for certain toxins. The presence of a specific toxin Bisphenol A in solution leads to displacement of the dye that manifests as a loss of fluorescence. The work presented here represents an interesting application of the de-aggregation induced fluorescence enhancement of dimeric carbocyanine dyes. Sub-micromolar levels of Bisphenol A can be detected using the aptamer-dimeric carbocyanine dye mediated approach. Further, the strategy is specific towards the detection of toxins based on the nucleic acid aptamers used in the assay. en_US
dc.description.statementofresponsibility Pallavi Chilka, Patlolla,Prathap Reddy and Bhaskar Datta
dc.format.extent vol. 57B, pp. 281-286,
dc.language.iso en en_US
dc.publisher NISCAIR-CSIR, India en_US
dc.subject Nucleic acid aptamers en_US
dc.subject Dimeric carbocyanine dye en_US
dc.subject G-quadruplex en_US
dc.subject Fluorescence sensing en_US
dc.subject Food borne toxins en_US
dc.title Dimeric carbocyanine dye and nucleic acid aptamer mediated detection of food borne toxin en_US
dc.type Article en_US
dc.relation.journal Indian Journal of Chemistry


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