High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer

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dc.contributor.author Bhoir, Siddhant
dc.contributor.author Shaik, Althaf
dc.contributor.author Thiruvenkatam, Vijay
dc.contributor.author Kirubakaran, Sivapriya
dc.date.accessioned 2018-03-27T06:17:53Z
dc.date.available 2018-03-27T06:17:53Z
dc.date.issued 2018-03
dc.identifier.citation Bhoir, Siddhant; Shaik, Althaf; Thiruvenkatam, Vijay and Kirubakaran, Sivapriya, “High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer”, Scientific Reports, DOI: 10.1038/s41598-018-22744-5, vol. 8, no. 1, Mar. 2018. en_US
dc.identifier.issn 2045-2322
dc.identifier.issn 2045-2322
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/3552
dc.identifier.uri http://dx.doi.org/10.1038/s41598-018-22744-5
dc.description.abstract Human Tousled-like kinases (TLKs) are highly conserved serine/threonine protein kinases responsible for cell proliferation, DNA repair, and genome surveillance. Their possible involvement in cancer via efficient DNA repair mechanisms have made them clinically relevant molecular targets for anticancer therapy. Innovative approaches in chemical biology have played a key role in validating the importance of kinases as molecular targets. However, the detailed understanding of the protein structure and the mechanisms of protein–drug interaction through biochemical and biophysical techniques demands a method for the production of an active protein of exceptional stability and purity on a large scale. We have designed a bacterial expression system to express and purify biologically active, wild-type Human Tousled-like Kinase 1B (hTLK1B) by co-expression with the protein phosphatase from bacteriophage λ. We have obtained remarkably high amounts of the soluble and homogeneously dephosphorylated form of biologically active hTLK1B with our unique, custom-built vector design strategy. The recombinant hTLK1B can be used for the structural studies and may further facilitate the development of new TLK inhibitors for anti-cancer therapy using a structure-based drug design approach. en_US
dc.description.statementofresponsibility by Siddhant Bhoir, Althaf Shaik, Vijay Thiruvenkatam and Sivapriya Kirubakaran
dc.format.extent vol. 8, no. 1
dc.language.iso en en_US
dc.publisher Nature Publishing Group en_US
dc.title High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer en_US
dc.type Article en_US
dc.relation.journal Scientific Reports


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