Surface immobilization of a short antimicrobial peptide (AMP) as an antibacterial coating

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dc.contributor.author Majhi, Sasmita
dc.contributor.author Arora, Ankita
dc.contributor.author Mishra, Abhijit
dc.date.accessioned 2019-06-19T11:12:57Z
dc.date.available 2019-06-19T11:12:57Z
dc.date.issued 2019-05
dc.identifier.citation Majhi, Sasmita; Arora, Ankita and Mishra, Abhijit, "Surface immobilization of a short antimicrobial peptide (AMP) as an antibacterial coating", Materialia, DOI: 10.1016/j.mtla.2019.100350, May 2019. en_US
dc.identifier.issn 2589-1529
dc.identifier.uri https://doi.org/10.1016/j.mtla.2019.100350
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/4530
dc.description.abstract Bacterial infections associated with commonly encountered abiotic surfaces are common threat to patient safety and health care. Antimicrobial peptides (AMPs) immobilization on such surfaces are considered as suitable approach towards addressing this clinical issue. In this study, we evaluate the antibacterial effect of immobilizing a short, pore-forming antimicrobial peptide, KLR (KLLLRLRKLLRR). KLR is covalently linked to polystyrene (PS) surfaces, via its C-terminal using EDC/NHS chemical coupling, and via its N-terminal using maleimide-thiol coupling with a Cysteine added to KLR (CKLLLRLRKLLRR) first. Physicochemical characterization of PS surfaces at each step of modification is carried out using atomic force microscopy, water contact angle measurements, and X-ray photoelectron spectroscopy. Antibacterial efficacy assays demonstrate that immobilized KLR shows excellent antibacterial activity against Gram-negative (E.coli ATCC 25922) and Gram-positive (S.aureus ATCC 25923) bacteria for both the coupling strategies. In addition, the AMP coatings possess no toxicity against mouse fibroblast cells (NIH 3T3).
dc.description.statementofresponsibility by Sasmita Majhi, Ankita Arora and Abhijit Mishra
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.title Surface immobilization of a short antimicrobial peptide (AMP) as an antibacterial coating en_US
dc.type Article en_US
dc.relation.journal Materialia


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