BSA-decorated magnesium nanoparticles for scavenging hydrogen peroxide from human hepatic cells

Show simple item record Shah, Juhi Pandya, Alok Goyal, Prateek Misra, Superb K. Singh, Sanjay 2020-04-07T09:58:46Z 2020-04-07T09:58:46Z 2020-03
dc.identifier.citation Shah, Juhi; Pandya, Alok; Goyal, Prateek; Misra, Superb K. and Singh, Sanjay, “BSA-decorated magnesium nanoparticles for scavenging hydrogen peroxide from human hepatic cells”, ACS Applied Nano Materials, DOI: 10.1021/acsanm.0c00088, vol. 3, no. 4, pp. 3355-3370, Mar. 2020. en_US
dc.identifier.issn 2574-0970
dc.description.abstract Inorganic nanomaterials have gathered significant attention due to their biological enzyme-like catalytic activities, which are currently being utilized to display excellent biomedical applications. Among these, inorganic nanomaterials exhibiting catalase enzyme-like properties are of particular interest because they can impart protection to mammalian cells from cellular hydrogen peroxide (H2O2) and other free radicals. In this study, we have investigated the utility of bovine serum albumin (BSA) conjugated magnesium nanoparticles (BSA-MgNPs) towards the protection of mammalian hepatic cells with depleted cellular catalase enzyme. We observed that BSA-MgNPs leads to the rapid breakdown of H2O2 and therefore, protect mammalian cells exposed from 3-Amino-1,2,4-triazole (3-AT), an irreversible catalase enzyme inhibitor. Results revealed that 3-AT induces oxidative stress by challenging the glutathione (GSH) and catalase enzyme, thus alter the cellular redox balance, which leads to the formation of reactive oxygen species (ROS) in human hepatocytes (WRL-68). Results showed that BSA-MgNPs impart protection to the catalase depleted hepatic cells. Expression analysis showed that BSA-MgNPs favourably modulate cellular antioxidant proteins/enzymes and genes such as catalase, thioredoxin reductase (TrxR), peroxiredoxin 6 (Prx6), and Hsp 70 (Heat shock protein). These results conclude that BSA-MgNPs impart protection to WRL-68 cells from acatalasia to display better cell survival as well as remarkable decrease in the intracellular ROS levels
dc.description.statementofresponsibility by Juhi Shah, Alok Pandya, Prateek Goyal, Superb K. Misra and Sanjay Singh
dc.language.iso en_US en_US
dc.publisher American Chemical Society en_US
dc.title BSA-decorated magnesium nanoparticles for scavenging hydrogen peroxide from human hepatic cells en_US
dc.type Article en_US
dc.relation.journal ACS Applied Nano Materials

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