Inducing endoplasmic reticulum stress in cancer cells by graphene oxide-based nanoparticles

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dc.contributor.author Pandey, Shalini
dc.contributor.author Nandi, Aditi
dc.contributor.author Basu, Sudipta
dc.contributor.author Ballav, Nirmalya
dc.date.accessioned 2020-08-28T11:47:34Z
dc.date.available 2020-08-28T11:47:34Z
dc.date.issued 2020-08
dc.identifier.citation Pandey, Shalini; Nandi, Aditi; Basu, Sudipta and Ballav, Nirmalya, “Inducing endoplasmic reticulum stress in cancer cells by graphene oxide-based nanoparticles”, Nanoscale Advances, DOI: 10.1039/D0NA00338G, vol. 2, no. 10, pp. 4887-4894, Aug. 2020. en_US
dc.identifier.issn 2516-0230
dc.identifier.uri https://doi.org/10.1039/D0NA00338G
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/5669
dc.description.abstract Endoplasmic reticulum is one of the vital organelles primarily involved in protein synthesis, folding, transport and lipid biosynthesis. However, in cancer cells its functions are dysregulated leading to ER stress. ER stress is now found to be closely associated with hallmarks of cancer and subsequently has emerged as an alluring target in cancer therapy. However, specific targeting of ER in cancer cell milieu remains a challenge. To address this, in this report we have engineered ER-targeted self-assembled 3D spherical graphene oxide nanoparticles (ER-GO-NPs) encompassing dual ER stress inducers, doxorubicin and cisplatin. DLS, FESEM and AFM techniques revealed that the nanoparticles were spherical in shape with sub 200 nm diameter. Confocal microscopy confirmed the specific homing of these ER-GO-NPs into the subcellular ER within 3 h. A combination of gel electrophoresis, confocal microscopy and flow cytometry studies revealed that these ER-GO-NPs induced ER stress mediated apoptosis in HeLa cells. Interestingly, the nanoparticles also activated autophagy which was inhibited through the cocktail treatment of ER-GO-NPs and chloroquine (CQ). At the same time these ER-GO-NPs were found to be efficient in prompting ER stress associated apoptosis in breast, lung and drug resistant triple negative breast cancer cell lines as well. We envision that these ER specific self-assembled graphene oxide nanoparticles can serve as a platform to exploit ER stress and its associated unfolded protein response (UPR) as a target resulting in promising therapeutic outcomes in cancer therapy.
dc.description.statementofresponsibility by Shalini Pandey, Aditi Nandi, Sudipta Basu and Nirmalya Ballav
dc.language.iso en_US en_US
dc.publisher Royal Society of Chemistry en_US
dc.title Inducing endoplasmic reticulum stress in cancer cells by graphene oxide-based nanoparticles en_US
dc.type Article en_US
dc.relation.journal Nanoscale Advances


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