Effect of binding-affinity and ATPase activity on the velocities of kinesins using Ratchet Models

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dc.contributor.author Mukherjee, Rupsha
dc.contributor.author Soppina, Pushpanjali
dc.contributor.author Patel, Nishaben
dc.contributor.author Soppina, Virupakshi
dc.contributor.author Rane, Kaustubh
dc.coverage.spatial United Kingdom
dc.date.accessioned 2022-02-11T08:02:48Z
dc.date.available 2022-02-11T08:02:48Z
dc.date.issued 2022-01
dc.identifier.citation Mukherjee, Rupsha; Soppina, Pushpanjali; Patel, Nishaben; Soppina, Virupakshi and Rane, Kaustubh, "Effect of binding-affinity and ATPase activity on the velocities of kinesins using Ratchet Models", Cell Biochemistry and Biophysics, DOI: 10.1007/s12013-021-01057-x, Jan. 2022. en_US
dc.identifier.issn 1085-9195
dc.identifier.issn 1559-0283
dc.identifier.uri https://doi.org/10.1007/s12013-021-01057-x
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/7504
dc.description.abstract We use two-state ratchet models containing single and coupled Brownian motors to understand the role of motor-microtubule binding, ATPase reaction rate and dimerisation on the translational velocities of Kinesin motors. We use model parameters derived from the experimental measurements on KIF1A, KIF13A, KIF13B, and KIF16B motors to compute velocities in ?m/s. We observe that both the models show the same trend in velocities (KIF1A?>?KIF13A?>?KIF13B?>?KIF16B) as the experimental results. However, the models significantly underpredict the velocities when compared with the experiments. The predictions of the coupled-motor model are closer to the experiments than those of the single-motor model. Our results indicate that the variation of ATPase reaction rate governs the trend in velocities for the above four motors. The variation of motor-microtubule binding affinity and the coupling strength between the motor domains may only have a secondary effect. More rigorous models that incorporate the power-stroke mechanism are necessary for better quantitative compliance with the experiments.
dc.description.statementofresponsibility by Rupsha Mukherjee, Pushpanjali Soppina, Nishaben Patel, Virupakshi Soppina and Kaustubh Rane
dc.language.iso en_US en_US
dc.publisher Springer en_US
dc.subject Binding-Affinity en_US
dc.subject ATPase Activity en_US
dc.subject Ratchet Model en_US
dc.subject Kinesin motors en_US
dc.subject Brownian motor en_US
dc.title Effect of binding-affinity and ATPase activity on the velocities of kinesins using Ratchet Models en_US
dc.type Article en_US
dc.relation.journal Cell Biochemistry and Biophysics

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