Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions

Show simple item record Sarpong, Kwabena Datta, Bhaskar 2014-03-16T14:00:55Z 2014-03-16T14:00:55Z 2012
dc.identifier.citation Sarpong, Kwabena and Datta, Bhaskar, “Nucleic acid binding chromophores as efficient indicators of aptamer target interactions”, Journal of Nucleic Acids, DOI: 10.1155/2012/247280, vol. 2012, no. 247280, pp. 1-7, 2012. en_US
dc.identifier.issn 2090-0201
dc.description.abstract The binding affinity and specificity of nucleic acid aptamers have made them valuable candidates for use as sensors in diagnostic applications. In particular, chromophore-functionalized aptamers offer a relatively simple format for detection and quantification of target molecules. We describe the use of nucleic-acid-staining reagents as an effective tool for detecting and signaling aptamer-target interactions. Aptamers varying in size and structure and targeting a range of molecules have been used in conjunction with commercially available chromophores to indicate and quantify the presence of cognate targets with high sensitivity and selectivity. Our assay precludes the covalent modification of nucleic acids and relies on the differential fluorescence signal of chromophores when complexed with aptamers with or without their cognate target. We also evaluate factors that are critical for the stability of the complex between the aptamer and chromophore in presence or absence of target molecules. Our results indicate the possibility of controlling those factors to enhance the sensitivity of target detection by the aptamers used in such assays. en_US
dc.description.statementofresponsibility by Kwabena Sarpong and Bhaskar Datta
dc.format.extent Vol. 2012, No. 247280, pp. 1-7
dc.language.iso en en_US
dc.publisher Hindawi Publishing Corporation en_US
dc.subject DNA en_US
dc.subject DNA synthesis en_US
dc.subject Fluorescent dye en_US
dc.subject Fluorescence spectroscopy en_US
dc.subject Molecular interaction en_US
dc.subject Nucleic acid structure en_US
dc.subject RNA en_US
dc.title Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions en_US
dc.type Article en_US
dc.relation.journal Journal of Nucleic Acids

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