Darban, ZenabZenabDarbanSingh, HemantHemantSinghSingh, UdishaUdishaSinghBhatia, DhirajDhirajBhatiaGaur, RamaRamaGaurKuddushi, MuzammilMuzammilKuddushiDhanka, MukeshMukeshDhankaShahabuddin, SyedSyedShahabuddin2025-08-312025-08-312024-01-0110.1016/j.ijbiomac.2023.1280192-s2.0-85177840878http://repository.iitgn.ac.in/handle/IITG2025/2647537952802Worldwide, burn wounds are severe health issues prone to bacterial infections and challenging to treat with traditional wound dressings. Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is required. Herein, aim to develop a biological macromolecules-based physically cross-linked gelatin/polyglyceryl stearate/graphene oxide (GPGO) hydrogel to treat burn wounds. Four sets of hydrogels were prepared by varying GO concentrations. FT-IR, FE-SEM, viscosity analysis, mechanical and thermal stability confirmed the successful preparation of hydrogels with desired properties. Further, β-carotene (0.5 mg/mL) was encapsulated in hydrogels to enhance the antioxidant activity, and a cumulative release as well as kinetics at pH 6.4 and 7.4 was performed. With an increase in GO concentration, hydrogels showed sustained release of β-carotene. Among all, GPGO-3 β hydrogel showed the highest antioxidant potency (57.75 %), hemocompatible (<5 %), cytocompatible (viable with NIH 3T3 cells), cell migration, proliferation, and in vitro wound healing. Also, GPGO-3 β hydrogel showed efficient antibacterial activity (%inhibition of 85.5 % and 80.2 % and zone of 11 mm and 9.8 mm against S. aureus and E. coli). These results demonstrated the ability of GPGO-3 β hydrogel as a promising candidate for burn wound healing applications.falseBurn wound | Gelatin | Polyglyceryl stearateArticle18790003January 202421128019arJournal21WOS:001126305500001