A., Singh, AnkurSingh, AnkurA.D.D.S., Devidas Bhatia, Dhiraj Devidas S.Devidas Bhatia, Dhiraj Devidas S.D.D.S.Shukla, A.K.2025-09-012025-09-0101237402589780125441650978032391607397801237252199780128220580978012370500601237064759780128033449012544173897803239002010091679X10.1016/bs.mcb.2022.01.0022-s2.0-85127327926https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127327926&doi=10.1016%2Fbs.mcb.2022.01.002&partnerID=40&md5=e7d898c26438a75175449d23d015e65dhttp://repository.iitgn.ac.in/handle/IITG2025/2941435623710From being genetic material to being exploited as an intelligent biomaterial, DNA has traveled a lot of scientific space of research and innovation. DNA can assemble into macromolecular polymeric networks based on sequences or by physically cross-linking their bulky lengthy strands. DNA is a polyanionic, hydrophilic, and polyelectrolytic natural biomaterial that can absorb large amounts of water mostly via H-bond interactions. The ability of DNA to attract water enables it form DNA-based hydrogels. DNA hydrogels offer many desirable qualities, making them an ideal choice as a desirable biomaterial for diverse applications. DNA Hydrogels show biodegradability, biocompatibility, modularity, non-toxicity, hydrophilicity, self-healing ability, and the ability to probe, program, and reprogram diverse biological systems. This chapter focuses on pure DNA-based hydrogels, their principles, and synthesis methods. We outlay various characterization tools and techniques followed by their biological applications and brief conclusion about their future employability for diverse biomedical applications. � 2022 Elsevier B.V., All rights reserved.EnglishbiomaterialDNApolymerwatergeneticshydrogelBiocompatible MaterialsHydrogelsPolymersWaterBook Chapter20227