Jana, PalashPalashJanaMukherjee, TarushyamTarushyamMukherjeeKhurana, RamanRamanKhuranaBarooah, NilotpalNilotpalBarooahSoppina, VirupakshiVirupakshiSoppinaMohanty, JyotirmayeeJyotirmayeeMohantyKanvah, SriramSriramKanvah2025-08-312025-08-312019-11-0110.1016/j.jphotochem.2019.1120622-s2.0-85071614073http://repository.iitgn.ac.in/handle/IITG2025/23154Two red-emitting water-soluble fluorophores bearing a coumarin scaffold were synthesized and their interactions with cucurbit[7]uril [CB7] were investigated. A significant red-shift in the absorption spectra showed the interaction of coumarin dyes with the carbonyl portals of CB[7]. The formation of supramolecular complex is substantiated by the chemical shift changes in ¹H-NMR and the thermodynamic parameters were evaluated by isothermal calorimetric titration (ITC). Upon encapsulation with the non-fluorescent CB host, significant enhancement of fluorescence quantum efficiency is observed. The large modulation of the emission properties of fluorophores in the presence of CB[7] indicates the formation of supramolecular complex. The advantageous enhanced quantum efficiency of the CB-dye complexes, is utilized to image live cells revealing localization in cellular membranes with greater photostability. These results based on inclusion complexes of the coumarin fluorophores with a non-toxic host CB[7] could lead to the design of efficient biological probes.falseCellular membrane imaging | Fluorescence probes | Host-guest interactions | Intramolecular charge transfer | Styryl coumarin dyesArticle1 November 201917112062arJournal20WOS:000487765700034