Singh, HemantHemantSinghYadav, InduInduYadavSheikh, Wajid MohammadWajid MohammadSheikhDan, AniruddhaAniruddhaDanDarban, ZenabZenabDarbanShah, Showkat AhmadShowkat AhmadShahMishra, Narayan ChandraNarayan ChandraMishraShahabuddin, SyedSyedShahabuddinHassan, ShabirShabirHassanBashir, Showkeen MuzamilShowkeen MuzamilBashirDhanka, MukeshMukeshDhanka2025-08-312025-08-312023-11-0110.1016/j.ijbiomac.2023.1263492-s2.0-85168758939http://repository.iitgn.ac.in/handle/IITG2025/2659237591426Biological macromolecules are excellent materials for wound dressing owing to their similar structure to the extracellular matrix and adjustable physicochemical properties. This research focuses on fabricating biological macromolecule-based hydrogel with desirable antibacterial, antioxidant, controlled drug release, cytocompatibility, and wound healing properties. Herein, different concentrations of nanoceria (NC) and flurbiprofen (FLU) drug-loaded gellan gum/gelatin (GG/Ge) based dual crosslinked (Ionic and EDC/NHS coupling) hydrogels were engineered. All fabricated hydrogels were hydrophilic, biodegradable, good strength, porous, antioxidant, hemocompatible and cytocompatible. Among all, hydrogel loaded with 500 μg/ml NC (GG/Ge/NC@FLU) exhibited desirable antioxidant, antibacterial (killed Staphylococcus aureus and Escherichia coli within 12 h), hemocompatible, cytocompatible, supports oxidative-stressed L929 cell growth and acted as a controlled release matrix for FLU, following Fickian diffusion, Peppas Sahlin and Korsmeyer-Peppas drug release models. Furthermore, nanocomposite hydrogel (GG/Ge/NC@FLU)-treated wounds of rats on day 14 demonstrated significantly higher collagen synthesis, nearly 100 % wound contractions, and efficiently decreased the expression of TNF-α and IL-1 while increasing the production of IL-10 and TNF-ß3, indicating antiinflammatory activity, and effectively reduced the expression of VEGF gene indicating effective angiogenesis than all other controls. In conclusion, the fabricated multifunctional GG/Ge/NC@FLU nanocomposite hydrogel shows promising potential for effectively treating full-thickness wound healing in a rat model.falseFull-thickness wound healing | Gelatin | Gellan gumArticle187900031 November 202338126349arJournal38WOS:001067847200001