Interaction of YOYO-1 with guanine-rich DNA.

Show simple item record

dc.contributor.author Ghosh Datta, Shohini
dc.contributor.author Reynolds, Christopher
dc.contributor.author Datta, Bhaskar
dc.contributor.author Goud, Yugender K.
dc.date.accessioned 2014-04-10T10:37:34Z
dc.date.available 2014-04-10T10:37:34Z
dc.date.issued 2014-07
dc.identifier.citation Ghosh Datta, Shohini; Reynolds, Christopher; Goud, Yugender K. and Datta, Bhaskar, “Interaction of YOYO-1 with guanine-rich DNA”, Journal of Biomolecular Structure and Dynamics, DOI: 10.1080/07391102.2013.807752, vol. 32, no. 7, Jul. 2014. en_US
dc.identifier.issn 0739-1102
dc.identifier.issn 1538-0254
dc.identifier.uri http://dx.doi.org/10.1080/07391102.2013.807752
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/1000
dc.description.abstract The oxazole homodimer YOYO-1 has served as a valuable tool for the detection and quantification of nucleic acids. While the base specificity and selectivity of binding of YOYO-1 has been researched to some extent, the effect of unorthodox nucleic acid conformations on dye binding has received relatively less attention. In this work, we attempt to correlate the quadruplex-forming ability of G-rich sequences with binding of YOYO-1. Oligonucleotides differing in the number of tandem G repeats, total length, and length of loop sequence were evaluated for their ability to form quadruplexes in presence of sodium (Na+) or potassium (K+) ions. The fluorescence behavior of YOYO-1 upon binding such G-rich sequences was also ascertained. A distinct correlation was observed between the strength and propensity of quadruplex formation, and the affinity of YOYO-1 to bind such sequences. Specifically, as exemplified by the oligonucleotides 5′-G4T2G4-3′ and 5′-G3TG3TG3-3′, sequences possessing longer G-rich regions and shorter loop sequences formed stronger quadruplexes in presence of K+ which translated to weaker binding of YOYO-1. The dependence of binding of YOYO-1 on sequence and structural features of G-rich DNA has not been explored previously and such studies are expected to aid in more effective interpretation of applications involving the fluorophore. en_US
dc.description.statementofresponsibility by Yugender K. Goud and Bhaskar Datta
dc.format.extent Vol. 32, No. 7, pp. 1155-1163
dc.language.iso en en_US
dc.publisher Taylor & Francis en_US
dc.subject Fluorescence enhancement of intercalating dyes en_US
dc.subject G-quadruplex en_US
dc.subject G-quartet dependence on monovalent cations en_US
dc.subject Nucleic acid staining reagents en_US
dc.subject Quadruplex topology en_US
dc.subject YOYO-1 en_US
dc.title Interaction of YOYO-1 with guanine-rich DNA. en_US
dc.type Article en_US
dc.relation.journal Journal of Biomolecular Structure and Dynamics


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account