P Transposable Elements in Drosophila and other Eukaryotic Organisms

Show simple item record

dc.contributor.author Majumdar, Sharmistha
dc.contributor.author Rio, Donald C.
dc.date.accessioned 2015-04-15T17:43:03Z
dc.date.available 2015-04-15T17:43:03Z
dc.date.issued 2015-04
dc.identifier.issn 2165-0497
dc.identifier.issn http://dx.doi.org/10.1128/microbiolspec.MDNA3-0004-2014
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/1671
dc.description.abstract P transposable elements were discovered in Drosophila as the causative agents of a syndrome of genetic traits called hybrid dysgenesis. Hybrid dysgenesis exhibits a unique pattern of maternal inheritance linked to the germline-specific small RNA piwi-interacting (piRNA) pathway. The use of P transposable elements as vectors for gene transfer and as genetic tools revolutionized the field of Drosophila molecular genetics. P element transposons have served as a useful model to investigate mechanisms of cut-and-paste transposition in eukaryotes. Biochemical studies have revealed new and unexpected insights into how eukaryotic DNA-based transposons are mobilized. For example, the P element transposase makes unusual 17nt-3′ extended double-strand DNA breaks at the transposon termini and uses guanosine triphosphate (GTP) as a cofactor to promote synapsis of the two transposon ends early in the transposition pathway. The N-terminal DNA binding domain of the P element transposase, called a THAP domain, contains a C2CH zinc-coordinating motif and is the founding member of a large family of animal-specific site-specific DNA binding proteins. Over the past decade genome sequencing efforts have revealed the presence of P element-like transposable elements or P element transposase-like genes (called THAP9) in many eukaryotic genomes, including vertebrates, such as primates including humans, zebrafish and Xenopus, as well as the human parasite Trichomonas vaginalis, the sea squirt Ciona, sea urchin and hydra. Surprisingly, the human and zebrafish P element transposase-related THAP9 genes promote transposition of the Drosophila P element transposon DNA in human and Drosophila cells, indicating that the THAP9 genes encode active P element “transposase” proteins. en_US
dc.description.statementofresponsibility by Sharmistha Majumdar and Donald C, Rio
dc.format.extent Vol.3, No.2
dc.language.iso en en_US
dc.publisher American Society for Microbiology en_US
dc.subject Drosophila en_US
dc.subject Eukaryotic Organisms en_US
dc.title P Transposable Elements in Drosophila and other Eukaryotic Organisms en_US
dc.type Article en_US
dc.relation.journal Microbiology Spectrum


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account