Gold-nanorod-enhanced FCS of fluorophores with high quantum yield in lipid bilayers

Show simple item record Pradhan, Biswajit Khatua, Saumyakanti Gupta, Ankur Aartsma, Thijs J. Canters, Gerard W. Orrit, Michel 2016-11-02T10:39:38Z 2016-11-02T10:39:38Z 2016-11
dc.identifier.citation Pradhan, Biswajit; Khatua, Saumyakanti; Gupta, Ankur; Aartsma, Thijs J.; Canters, Gerard W. and Orrit, Michel, “Gold-nanorod-enhanced fluorescence correlation spectroscopy of fluorophores with high quantum yield in lipid bilayers”, The Journal of Physical Chemistry C, DOI: 10.1021/acs.jpcc.6b07875, vol. 120, no. 45, pp. 25996-26003, Nov. 2016.
dc.identifier.issn 1932-7447
dc.identifier.issn 1932-7455
dc.description.abstract Plasmonic fluorescence enhancement is used to study fluorescence correlation spectroscopy (FCS) at higher concentrations than in regular diffraction-limited FCS experiments. Previous studies suffered from sticking to the substrate and were performed mainly with poorly emitting dyes. A lipid bilayer forms a passivating surface preventing sticking of the dye or the protein and allows for specific anchoring of probe molecules. For dyes with high quantum yields, the fluorescence background of unenhanced molecules is high, and the fluorescence enhancement is weak, less than about 10. Nonetheless, we show that FCS is possible at micromolar concentrations of the probe molecule. Enhanced FCS is recorded by selecting signals on the basis of their shortened lifetime. This selection enhances the contrast of the correlation by more than an order of magnitude. The lipid bilayer can be used to anchor biomolecules and perform enhanced FCS, as we show for a dye-labeled protein. en_US
dc.description.statementofresponsibility by Biswajit Pradhan, Saumyakanti Khatua, Ankur Gupta, Thijs J. Aartsma, Gerard W. Canters, and Michel Orrit
dc.format.extent Vol. 120 no. 45, pp. 25996–26003
dc.language.iso en_US en_US
dc.publisher American Chemical Society en_US
dc.subject Fluorescence
dc.subject Vesicles
dc.subject Dyes and pigments
dc.subject Molecules
dc.title Gold-nanorod-enhanced FCS of fluorophores with high quantum yield in lipid bilayers en_US
dc.type Article en_US
dc.relation.journal Journal of Physical Chemistry C

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