Interaction of a dimeric carbocyanine dye aggregate with bovine serum albumin in non-aggregated and aggregated forms

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dc.contributor.author Reddy Patlolla, Prathap
dc.contributor.author Desai, Nakshi Nayan
dc.contributor.author Gupta, Sharad
dc.contributor.author Datta, Bhaskar
dc.date.accessioned 2018-11-16T06:05:55Z
dc.date.available 2018-11-16T06:05:55Z
dc.date.issued 2018-10
dc.identifier.citation Reddy Patlolla, Prathap; Desai, Nakshi; Gupta, Sharad and Datta, Bhaskar, "Interaction of a dimeric carbocyanine dye aggregate with bovine serum albumin in non-aggregated and aggregated forms", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, DOI: 10.1016/j.saa.2018.10.048, Oct. 2018. en_US
dc.identifier.issn 1386-1425
dc.identifier.uri https://doi.org/10.1016/j.saa.2018.10.048
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/4005
dc.description.abstract The interaction of fluorescent dyes with serum proteins has garnered significant interest owing to potential for non-covalent labeling and imaging applications. In this work, dimeric benzothiazole-based trimethine cyanine dyes are synthesized and their interaction with bovine serum albumin studied. The dimeric cyanine dyes mainly exist as H-dimers and H-aggregates in aqueous solution. A combination of absorbance, fluorescence, circular dichroism spectroscopy and atomic force and fluorescence microscopy indicate the formation of dye-BSA complexes. Binding of one of the dimeric dyes on BSA with a Ka of 1.49 × 105 M−1 results in disruption of dye self-aggregates and unfolding of the dyes into the monomeric or open conformation. Fluorescence enhancement experienced by the dimeric dyes upon interaction with BSA is superior to that registered by Thioflavin T. Surfactant SDS has been used to further tune the self-aggregation of the dimeric dye resulting in a 200-fold fluorescence enhancement in presence of BSA. Interaction of a dimeric dye with BSA under conditions favoring protein aggregation is found to result in faster dye binding and the resulting fluorescence enhancement is easily visualized by fluorescence microscopy. The interaction of a dimeric cyanine dye aggregate with BSA is promising for non-covalent labeling applications in sharp contrast to the monomeric dye counterpart.
dc.description.statementofresponsibility by Prathap Reddy Patlolla, Nakshi Desai, Sharad Gupta and Bhaskar Datta
dc.format.extent vol..209, pg.256-263
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Dimeric carbocyanine dye en_US
dc.subject bovine serum albumin en_US
dc.subject non-aggregated form en_US
dc.subject aggregated form en_US
dc.title Interaction of a dimeric carbocyanine dye aggregate with bovine serum albumin in non-aggregated and aggregated forms en_US
dc.type Article en_US
dc.relation.journal Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy


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