Investigation of nanoparticle immobilized cellulase: nanoparticle identity, linker length and polyphenol hydrolysis

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dc.contributor.author Kumar, Sanjay
dc.contributor.author Morya, Vinod
dc.contributor.author Gadhavi, Joshna
dc.contributor.author Vishnoi, Anjani
dc.contributor.author Singh, Jaskaran
dc.contributor.author Datta, Bhaskar
dc.date.accessioned 2019-06-19T11:12:57Z
dc.date.available 2019-06-19T11:12:57Z
dc.date.issued 2019-05
dc.identifier.citation Kumar, Sanjay; Morya, Vinod; Gadhavi, Joshna; Vishnoi, Anjani; Singh, Jaskaran and Datta, Bhaskar, "Investigation of nanoparticle immobilized cellulase: nanoparticle identity, linker length and polyphenol hydrolysis", Heliyon, DOI: 10.1016/j.heliyon.2019.e01702, vol. 5, no. 5 en_US
dc.identifier.issn 2405-8440
dc.identifier.uri https://doi.org/10.1016/j.heliyon.2019.e01702
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/4542
dc.description.abstract Cellulase containing nanobiocatalysts have been useful as an extraction tool based on their ability to disrupt plant cell walls. In this work, we investigate the effect of nanoparticle composition and chemical linkage towards immobilized cellulase activity. Cellulase nanoconstructs have been prepared, characterized and compared for their loading efficiencies with standard assays and enzyme kinetics and correlate well with the cognate loading efficiencies. Application of the cellulase-immobilized nanoparticles on onion skins results in release of a distinctive composition of polyphenols. The aglycosidic form of quercetin is the dominant product of onion skin hydrolysis affected by cellulase nanobiocatalysts. Chitosan-coated iron oxide nanoparticles with APTES-conjugated cellulase are found to be most effective for polyphenol release and for transformation of glycosidic to aglycosidic form of quercetin. These results shed light on the activity of immobilized cellulase beyond their role in cell wall disruption and are important for the practical application of cellulase nanobiocatalysts.
dc.description.statementofresponsibility by Sanjay Kumar, Vinod Morya, Joshna Gadhavi, Anjani Vishnoi, Jaskaran Singh and Bhaskar Datta
dc.format.extent vol. 5, no. 5
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Biochemistry en_US
dc.subject Biotechnology en_US
dc.subject Bioengineering en_US
dc.title Investigation of nanoparticle immobilized cellulase: nanoparticle identity, linker length and polyphenol hydrolysis en_US
dc.type Article en_US
dc.relation.journal Heliyon


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