Nanobiocatalyst facilitated aglycosidic quercetin as a potent inhibitor of tau protein aggregation

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dc.contributor.author Kumar, Sanjay
dc.contributor.author Guru, Krishnakumar Viswanathan
dc.contributor.author Morya, Vinod
dc.contributor.author Gupta, Sharad
dc.contributor.author Datta, Bhaskar
dc.date.accessioned 2019-08-06T09:37:22Z
dc.date.available 2019-08-06T09:37:22Z
dc.date.issued 2019-10
dc.identifier.citation Kumar, Sanjay; Guru Krishnakumar, V.; Morya, Vinod; Gupta, Sharad and Datta, Bhaskar, "Nanobiocatalyst facilitated aglycosidic quercetin as a potent inhibitor of tau protein aggregation", International Journal of Biological Macromolecules, DOI: 10.1016/j.ijbiomac.2019.07.081, vol. 138, pp. 168-180, Oct. 2019. en_US
dc.identifier.issn 0141-8130
dc.identifier.uri https://doi.org/10.1016/j.ijbiomac.2019.07.081
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/4630
dc.description.abstract Polyphenols have been suggested as potential therapeutic agents for the treatment of amyloidogenic diseases. In this work, we evaluate quercetin-rich onion extract for its ability to inhibit tau fibrillization. Considering the presence of polyphenols in multiple glycosidic and aglycosidic forms, a nanobiocatalyst-mediated approach has been used to extract quercetin from onion skins. The nanobiocatalysts facilitate greater release of quercetin compared to the use of free enzymes. Atomic force microscopy and fluorescence microscopy show that quercetin possesses a novel inhibitory character on tau-fibril aggregation. In contrast, quercetin-diglucoside does not have an inhibitory effect. Molecular Dynamics simulations reveal conformational changes in tau protein upon interaction with quercetin due to specific hydrogen bonding and hydrophobic interactions. The resulting conformational stability of tau monomer reduces propensity of the protein to aggregate. The ability of quercetin to inhibit tau fibrillization expands the paradigm for application of bioactive polyphenols.
dc.description.statementofresponsibility by Sanjay Kumar, V. Guru Krishnakumar, Vinod Morya, Sharad Gupta and Bhaskar Datta
dc.format.extent vol. 138, pp. 168-180
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.title Nanobiocatalyst facilitated aglycosidic quercetin as a potent inhibitor of tau protein aggregation en_US
dc.type Article en_US
dc.relation.journal International Journal of Biological Macromolecules


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