High yield expression of recombinant CD151 in E.coli & a structural insight into cholesterol binding domain

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dc.contributor.author Purushothaman, Gayathri
dc.contributor.author Thiruvenkatam, Vijay
dc.date.accessioned 2019-09-18T10:12:53Z
dc.date.available 2019-09-18T10:12:53Z
dc.date.issued 2019
dc.identifier.citation Thiruvenkatam, Vijay, "High yield expression of recombinant CD151 in E.coli & a structural insight into cholesterol binding domain", Molecular Biotechnology, DOI: 10.1007/s12033-019-00212-3, vol. 61, 12, pp. 905-915, Sep. 2019. en_US
dc.identifier.issn 1073-6085
dc.identifier.issn 1559-0305
dc.identifier.uri https://doi.org/10.1007/s12033-019-00212-3
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/4828
dc.description.abstract CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of structural information. By considering the difficulties in expressing the membrane protein in E. coli, herein we introduce the strategic design for the effective expression of recombinant CD151 protein in E. coli with high yield, that would aid for the structural studies. CD151 having four transmembrane domain (TMD’s) along with small and a large extracellular loop (LEL) is constructed in parts to enhance the soluble expression of the protein attached with fusion tag. This has led to the high yield of the recombinant CD151 protein in the designed constructs. The recombinant CD151 protein is characterized and confirmed by western blot, CD and Mass peptide fingerprint. The molecular dynamics simulations (MDS) for the full-length CD151 shows conformational changes in the LEL of the protein in the presence and absence of cholesterol and indicate the certainty of closed and open conformation of CD151 based on cholesterol binding. The MDS results have led to the understanding of the possible underlying mechanism for the activation of the CD151 protein.
dc.description.statementofresponsibility by Gayathri Purushothaman and Vijay Thiruvenkatam
dc.format.extent vol. 61, 12, pp. 905-915
dc.language.iso en_US en_US
dc.publisher Springer en_US
dc.subject Cholesterol binding domain
dc.subject Molecular dynamics simulation
dc.subject Recombinant expression
dc.subject Construct design
dc.subject CD151
dc.subject Transmembrane protein
dc.title High yield expression of recombinant CD151 in E.coli & a structural insight into cholesterol binding domain en_US
dc.type Article en_US
dc.relation.journal Molecular Biotechnology


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