Exploring potential of glass surface immobilized short antimicrobial peptide (AMP) as antibacterial coatings

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dc.contributor.author Majhi, Sasmita
dc.contributor.author Mishra, Abhijit
dc.coverage.spatial United States of America
dc.date.accessioned 2012-09-26T07:22:31Z
dc.date.available 2012-09-26T07:22:31Z
dc.date.issued 2022-01
dc.identifier.citation Majhi, Sasmita and Mishra, Abhijit, “Exploring potential of glass surface immobilized short antimicrobial peptide (AMP) as antibacterial coatings”, Materialstoday: Proceedings, DOI: 10.1016/j.matpr.2021.07.061, vol. 49, pp. 1367-1377, Jan. 2022. en_US
dc.identifier.issn 2214-7853
dc.identifier.uri https://doi.org/10.1016/j.matpr.2021.07.061
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/6647
dc.description.abstract Bacterial infections associated with biomedical devices and implants pose serious clinical threat to human health and medical sector. Antimicrobial peptides (AMPs) based coatings are promising candidate to prevent these infections due to broad spectrum antimicrobial activity, reduced bacterial resistance propensity, and low cytotoxicity. In this study, an in-house designed peptide, KLR (KLLLRLRKLLRR), is immobilized onto glass substrates and its antibacterial activity against E. coli and S. aureus is studied. The effect of orientation of the peptide on the antibacterial activity is also tested by tethering the peptide through its C-terminus, using EDC/NHS coupling, and N-terminus, using maleimide-thiol chemical coupling. To confirm successful modification in all the sequential steps, X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurements, and atomic force microscopy (AFM) is used. Antibacterial assays reveal that peptide-modified surfaces exhibit excellent antibacterial activity against E. coli (Gram-negative bacteria) but were ineffective in inhibiting S. aureus (Gram-positive bacteria). Furthermore, confocal studies with dye encapsulated giant unilamellar vesicles (GUVs) show that immobilized KLR induces dye leakage from the vesicles indicating pore-forming action mechanism of immobilized peptide. These peptide coated surfaces exhibit no cytotoxicity towards mammalian cells. Thus, these short peptides can be synthesized easily and cost effectively, opening an opportunity to be new antibacterial surface coating agents.
dc.description.statementofresponsibility by Sasmita Majhi and Abhijit Mishra
dc.format.extent vol. 49, pp. 1367-1377
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject Glass en_US
dc.subject Antimicrobial peptide en_US
dc.subject Antibacterial coating en_US
dc.subject Immobilization en_US
dc.subject EDC/NHS coupling en_US
dc.subject Maleimide-thiol coupling en_US
dc.title Exploring potential of glass surface immobilized short antimicrobial peptide (AMP) as antibacterial coatings en_US
dc.type Article en_US
dc.relation.journal Materials Today: Proceedings

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