Novel dual labelled nanoprobes for nanosafety studies: quantification and imaging experiment of CuO nanoparticles in C. elegans

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dc.contributor.author Butreddy, Pravalika
dc.contributor.author Chakraborty, Swaroop
dc.contributor.author Soppina, Pushpanjali
dc.contributor.author Behera, Rakesh
dc.contributor.author Soppina, Virupakshi
dc.contributor.author Misra, Superb K.,
dc.coverage.spatial United Sates of America
dc.date.accessioned 2012-09-19T16:39:14Z
dc.date.available 2012-09-19T16:39:14Z
dc.date.issued 2022-01
dc.identifier.citation Butreddy, Pravalika; Chakraborty, Swaroop; Soppina, Pushpanjali; Behera, Rakesh; Soppina, Virupakshi and Misra, Superb K., "Novel dual labelled nanoprobes for nanosafety studies: quantification and imaging experiment of CuO nanoparticles in C. elegans", Chemosphere, DOI: 10.1016/j.chemosphere.2021.131698, vol. 286, Jan. 2022. en_US
dc.identifier.issn 0045-6535
dc.identifier.uri https://doi.org/10.1016/j.chemosphere.2021.131698
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/6801
dc.description.abstract Metal oxide nanoparticles have been extensively studied for their toxicological impacts. However, accurate tracing/quantification of the nanomaterials and their biological responses are difficult to measure at low concentrations. To overcome the challenge, we developed a dual-labelling technique of CuO nanoparticles with a stable isotope of 65Cu, and with rhodamine dye. In vivo experiments on C. elegans were performed using natural feeding of Rhodamine B isothiocyanate-(3 aminopropyl) triethoxysilane functionalized 65CuO nanoprobes (RBITC-APTES@65CuO) (size = 7.41 ± 1 nm) within the range of Predicted Environmental Concentration (PEC) of CuO nanoparticles in soil and sediments. Fluorescence emission (570 nm) was detected in the lumen of the intestine and the pharynx of C. elegans with no impact of nanoparticle exposure on the brood size and life span of worms. The ingested fluorescent labelled RBITC-APTES@65CuO nanoprobes did not enter the reproductive system and were distributed in the alimentary canal of C. elegans. Strong fluorescent signals from the ingested RBITC-APTES@65CuO nanoprobes were achieved even after 24 h of exposure demonstrating the high stability of these nanoprobes in vivo. The net accumulation measured of 65Cu in C. elegans after background subtraction was 0.001 μg mg−1 (3.52 %), 0.005 μg mg−1 (1.76 %) and 0.024 μg mg−1 (1.69 %) for an exposure concentration of 0.0284 μg mg−1, 0.284 μg mg−1, and 1.42 μg mg−1 of 65Cu, respectively. Using C. elegans as a model organism, we demonstrated that RBITC-APTES tagged 65CuO nanoparticles acted as novel nanoprobes for measuring the uptake, accumulation, and biodistribution through quantification and imaging the nanoprobes at a very low exposure concentration (65CuO concentration: 0.033 μg mg−1).
dc.description.statementofresponsibility by Pravalika Butreddy, Swaroop Chakraborty, Pushpanjali Soppina, Rakesh Behera, Virupakshi Soppina and Superb K. Misra
dc.format.extent vol. 286
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject Stable isotope en_US
dc.subject Fluorescent labelling en_US
dc.subject Nanosafety en_US
dc.subject CuO nanoparticles en_US
dc.subject Tracing and imaging en_US
dc.subject Fate and accumulation en_US
dc.title Novel dual labelled nanoprobes for nanosafety studies: quantification and imaging experiment of CuO nanoparticles in C. elegans en_US
dc.type Article en_US
dc.relation.journal Chemosphere


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