Effect of antimicrobial peptide (AMP)-tethered stainless steel surfaces on the bacterial membrane

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dc.contributor.author Majhi, Sasmita
dc.contributor.author Peddiraju, Vivek Chaitanya
dc.contributor.author Mishra, Abhijit
dc.coverage.spatial United States of America
dc.date.accessioned 2012-09-26T18:43:25Z
dc.date.available 2012-09-26T18:43:25Z
dc.date.issued 2021-08
dc.identifier.citation Majhi, Sasmita; Peddiraju, Vivek Chaitanya and Mishra, Abhijit, "Effect of antimicrobial peptide (AMP)-tethered stainless steel surfaces on the bacterial membrane", Materialstoday: Chemistry, DOI: 10.1016/j.mtchem.2021.100541, vol. 21, Aug. 2021. en_US
dc.identifier.issn 2468-5194
dc.identifier.uri https://doi.org/10.1016/j.mtchem.2021.100541
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/6821
dc.description.abstract Bacterial colonization leading to biofilm formation on surfaces induces industry-related as well as health care-associated infections worldwide. Emerging antibiotic-resistant microbes and related persistent infections due to adherent biofilm formation on surfaces have urged the need of effective alternative solutions to eradicate prevailing problems. Antimicrobial peptides are considered as potential candidates with distinguished characteristics, namely, broad-spectrum antimicrobial activity, low propensity toward pathogen resistance, and low immune response. In this study, we immobilize an in-house-designed peptide, KLLLRLRKLLRR (KLR), using a 2-step functionalization strategy onto stainless steel (SS) surfaces. SS is amino-silanized using (3-aminopropyl) triethoxysilane followed by tethering of KLR on it via formation of the amide bond. KLR-coated SS surfaces show nearly 95-100% reduction in bacterial colonization in vitro as obtained from antibacterial susceptibility testing while being non-toxic to mammalian cells. The coating strategy does not affect the microstructure of the SS surfaces. These findings demonstrate that this tethering process is able to produce excellent antibacterial surfaces.
dc.description.statementofresponsibility by Sasmita Majhi, Vivek Chaitanya Peddiraju and Abhijit Mishra
dc.format.extent vol. 21
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject KLR en_US
dc.subject Antibacterial activity en_US
dc.subject Cytocompatibility en_US
dc.subject Covalent immobilization en_US
dc.subject EDC/NHS chemistry en_US
dc.title Effect of antimicrobial peptide (AMP)-tethered stainless steel surfaces on the bacterial membrane en_US
dc.type Article en_US
dc.relation.journal Materialstoday: Chemistry


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