Abstract:
We report the synthesis and characterization of the structures formed by self-assembly of 4-chloro-2(3H)-benzothiazolone (CBT) into panchromatic fibres and their application in cellular imaging. The aggregationproperties of the synthesized compound were studied extensively under different solvents and concentrationsand their morphologies examined at a supramolecular level by various microscopic techniques such as atomicforce microscopy (AFM), fluorescence microscopy, and optical microscopy. Interestingly, the self-assembledstructures formed by CBT reveal panchromatic emission properties and show blue, green, and red fluorescenceunder different excitation wavelengths. The mechanism of structure formation of the self-assemblies was studied through different techniques such as concentration-dependent 1H-NMR, ATR-FTIR, UV-visiblespectroscopy, and fluorescence spectroscopy. Finally, the utility of CBT for cell imaging applications wasdemonstrated and it can be noted that CBT was efficiently taken up by mammalian cells and the cells revealedpanchromatic emission in the blue, green, and red channels. The intensities of the fluorescence observed wereblue 4 green 4 red and the dye interestingly does not exhibit any fluorescence quenching