Absolute non-invasive measurement of CO2 mole fraction emitted by E. coli and S. aureus using calibration-free 2f WMS applied to a 2004 nm VCSEL

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dc.contributor.author A. S., Zarin
dc.contributor.author Chakraborty, Arup Lal
dc.contributor.author Upadhyay, Abhishek
dc.date.accessioned 2017-04-26T11:05:13Z
dc.date.available 2017-04-26T11:05:13Z
dc.date.issued 2017-06
dc.identifier.citation A. S., Zarin; Chakraborty, Arup and Upadhyay, Abhishek, “Absolute non-invasive measurement of CO2 mole fraction emitted by E. coli and S. aureus using calibration-free 2f WMS applied to a 2004 nm VCSEL ”, Optics Letters, DOI: 10.1364/OL.42.002138, Jun. 2017.
dc.identifier.issn ‎0146-9592
dc.identifier.issn 1539-4794
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/2886
dc.identifier.uri http://dx.doi.org/10.1364/OL.42.002138
dc.description.abstract We report the first demonstration of accurate real-time non-invasive measurement of the absolute cumulative mole fraction of metabolic carbon dioxide emitted by Escherichia coli and Staphylococcus aureus over a period of several hours of their life cycles using a recentlydeveloped calibration-free wavelength modulation spectroscopy technique. A 1mW vertical cavity surface emitting laser is used to interrogate a single rotational vibrational absorption line of carbon dioxide at 2003.5 nm. The measurements are immune to laser intensity fluctuations and variable optical coupling that is inevitable in such free-space coupled experiments that run over 10-18 hours. The cumulative carbon dioxide mole fraction follows the characteristic modified Gompertz model that is typical of bacterial growth in batch cultures. The characteristics growth parameters are extracted from this curve. The technique can be readily extended to study multiple volatile organic compounds that bacteria are known to emit. en_US
dc.description.statementofresponsibility by Zarin A. S., Arup Lal Chakraborty and Abhishek Upadhyay
dc.format.extent Vol. 42, Issue 11, pp. 2138-2141
dc.language.iso en_US en_US
dc.publisher Optical Society of America en_US
dc.subject OCIS codes: (300.6260) Spectroscopy, diode lasers en_US
dc.subject OCIS code: (300.6380) Spectroscopy, modulation en_US
dc.subject OCIS Code: (280.1415) Biological sensing and sensors en_US
dc.subject OCIS Code: (140.7260) Vertical cavity surface emitting lasers en_US
dc.subject OCIS Code: (170.4090) en_US
dc.subject Wavelength modulation spectroscopy en_US
dc.subject Escherichia coli en_US
dc.subject Staphylococcus aureus en_US
dc.title Absolute non-invasive measurement of CO2 mole fraction emitted by E. coli and S. aureus using calibration-free 2f WMS applied to a 2004 nm VCSEL en_US
dc.type Article en_US
dc.relation.journal Optics Letters


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