Decay of SARS-CoV-2 RNA along the wastewater treatment outfitted with Upflow Anaerobic Sludge Blanket (UASB) system evaluated through two sample concentration techniques

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dc.contributor.author Kumar, Manish
dc.contributor.author Kuroda, Keisuke
dc.contributor.author Patel, Arbind Kumar
dc.contributor.author Patel, Nidhi
dc.contributor.author Bhattacharya, Prosun
dc.contributor.author Joshi, Madhvi
dc.contributor.author Joshi, Chaitanya G.
dc.date.accessioned 2020-10-09T09:55:55Z
dc.date.available 2020-10-09T09:55:55Z
dc.date.issued 2021-02
dc.identifier.citation Kumar, Manish; Kuroda, Keisuke; Patel, Arbind Kumar; Patel, Nidhi; Bhattacharya, Prosun; Joshi, Madhvi and Joshi, Chaitanya G., "Decay of SARS-CoV-2 RNA along the wastewater treatment outfitted with Upflow Anaerobic Sludge Blanket (UASB) system evaluated through two sample concentration techniques", Science of The Total Environment, DOI: 10.1016/j.scitotenv.2020.142329, vol. 754, Feb. 2021. en_US
dc.identifier.issn 0048-9697
dc.identifier.issn 1879-1026
dc.identifier.uri https://doi.org/10.1016/j.scitotenv.2020.142329
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/5754
dc.description.abstract For the first time, we present, i) an account of decay in the genetic material loading of SARS-CoV-2 during Upflow Anaerobic Sludge Blanket (UASB) treatment of wastewater, and ii) comparative evaluation of polyethylene glycol (PEG), and ultrafiltration as virus concentration methods from wastewater for the quantification of SARS-CoV-2 genes. The objectives were achieved through tracking of SARS-CoV-2 genetic loadings i.e. ORF1ab, N and S protein genes on 8th and 27th May 2020 along the wastewater treatment plant (106000 m3 million liters per day) equipped with UASB system in Ahmedabad, India. PEG method performed better in removing materials inhibiting RT-qPCR for SARS-CoV-2 gene detection from the samples, as evident from constant and lower CT values of control (MS2). Using the PEG method, we found a reduction >1.3 log10 reduction in SARS-CoV-2 RNA abundance during UASB treatment, and the RNA was not detected at all in the final effluent. The study implies that i) conventional wastewater treatment systems is effective in SARS-CoV-2 RNA removal, and ii) UASB system significantly reduces SARS-CoV-2 genetic loadings. Finally, PEG method is recommended for better sensitivity and inhibition removal during SARS-CoV-2 RNA quantification in wastewater.
dc.description.statementofresponsibility by Manish Kumar, Keisuke Kuroda, Arbind Kumar Patel, Nidhi Patel, Prosun Bhattacharya, Madhvi Joshi and Chaitanya G.Joshid
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject SARS-CoV-2 en_US
dc.subject COVID-19 en_US
dc.subject Environmental surveillance en_US
dc.subject Virus concentration methods en_US
dc.subject UASB en_US
dc.subject Wastewater based epidemiology en_US
dc.title Decay of SARS-CoV-2 RNA along the wastewater treatment outfitted with Upflow Anaerobic Sludge Blanket (UASB) system evaluated through two sample concentration techniques en_US
dc.type Article en_US
dc.relation.journal Science of The Total Environment


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