Effectiveness of oil-layered albumin microbubbles produced using microfluidic T-junctions in series for in-vitro inhibition of tumor cells

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dc.contributor.author Khan, Aaqib H.
dc.contributor.author Jiang, Xinyue
dc.contributor.author Surwase, Swarupkumar
dc.contributor.author Gultekinoglu, Merve
dc.contributor.author Bayram, Cem
dc.contributor.author Sathisaran, Indumathi
dc.contributor.author Bhatia, Dhiraj
dc.contributor.author Ahmed, Jubair
dc.contributor.author Wu, Bingjie
dc.contributor.author Ulubayram, Kezban
dc.contributor.author Edirisinghe, Mohan
dc.contributor.author Dalvi, Sameer V.
dc.date.accessioned 2020-11-05T06:07:18Z
dc.date.available 2020-11-05T06:07:18Z
dc.date.issued 2020-09
dc.identifier.citation Khan, Aaqib H.; Jiang, Xinyue; Surwase, Swarupkumar; Gultekinoglu, Merve; Bayram, Cem; Sathisaran, Indumathi; Bhatia, Dhiraj; Ahmed, Jubair; Wu, Bingjie; Ulubayram, Kezban; Edirisinghe, Mohan and Dalvi, Sameer V., "Effectiveness of oil-layered albumin microbubbles produced using microfluidic T-junctions in series for in-vitro inhibition of tumor cells", Langmuir, DOI: 10.1021/acs.langmuir.0c01557, Sep. 2020. en_US
dc.identifier.issn 0743-7463
dc.identifier.issn 1520-5827
dc.identifier.uri https://doi.org/10.1021/acs.langmuir.0c01557
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/5839
dc.description.abstract This work focuses on evaluation of the effectiveness of double layered microbubbles loaded with doxorubicin and curcumin for cell invasion arrest from 3D spheroid models of triple negative breast cancer (TNBC), MDA-MB-231 cell line. Albumin microbubbles coated in drug-laden oil layer were synthesized using a new method of connecting two microfluidic T-mixers in series. Double-layered microbubbles thus produced consist of an innermost core of nitrogen gas encapsulated in an aqueous layer of bovine serum albumin (BSA) which in turn, is coated with an outer layer of silicone oil. In order to identify the process conditions leading to the formation of double-layered microbubbles, a regime map was constructed based on Capillary numbers for aqueous and oil phases. The microbubble formation regime transitions from double-layered to single layer microbubbles and then to formation of single oil droplets upon gradual change in flow rates of aqueous and oil phases. In-vitro dissolution studies of double-layered microbubbles in an air-saturated environment indicated that a complete dissolution of such bubbles produces an oil droplet devoid of gas bubble. Incorporation of doxorubicin and curcumin was found to produce a synergistic effect, which resulted in higher cell deaths in 2D monolayers of TNBC cells and inhibition of cell proliferation from 3D spheroid models of TNBC cells compared to control.
dc.description.statementofresponsibility by Aaqib Khan, Xinyue Jiang, Swarup Surwase, Merve Gultekinoglu, Cem Bayram, Indumathi Sathisaran, Dhiraj Bhatia, Jubair Ahmed, Bingjie Wu, Kezban Ulubayram, Mohan Edirisinghe, and Sameer Vishvanath Dalvi
dc.language.iso en_US en_US
dc.publisher American Chemical Society en_US
dc.title Effectiveness of oil-layered albumin microbubbles produced using microfluidic T-junctions in series for in-vitro inhibition of tumor cells en_US
dc.type Article en_US
dc.relation.journal Langmuir


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