Acetone-butanol-ethanol fermentation analysis using only high performance liquid chromatography

Show simple item record Kumar, Manish Saini, Supreet Gayen, Kalyan 2014-03-17T09:56:01Z 2014-03-17T09:56:01Z 2014
dc.identifier.citation Kumar, Manish; Saini, Supreet and Gayen, Kalyan, “Acetone-Butanol-Ethanol (ABE) fermentation analysis using only high performance liquid chromatography”, Analytical Methods, DOI: 10.1039/c3ay41717d, vol. 6, no. 3, 2014. en_US
dc.identifier.issn 1759-9679
dc.description.abstract Currently, sample analysis of Acetone-butanol-ethanol (ABE) fermentation in Clostridium acetobutylicum is performed through the simultaneous use of both High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). In this study, a novel method was developed for the quantification of substrate (glucose) and products (acetic acid, ethanol, butyric acid, acetone, and butanol) of ABE fermentation using only HPLC. The most favorable characterization of peaks of tested compounds were observed in a refractive index (RI) detector maintaining a flow rate of 0.5 ml min−1 with a column temperature of 30 °C. However, an overlap between butyric acid and acetone peaks was detected, and therefore a methodology was developed accounting for respective peak heights for the quantification of these compounds. During validation of the method, linear regression analysis of the calibration plot illustrated that there was a good linear relationship (correlation coefficient R2 > 0.9981) between peak area and concentration in the range of 0.31–5.0 mg ml−1. The quantitative recoveries of tested components were very close in range, 97.99–103.46%, and Relative Standard Deviation (RSD) values were lower than 4.90%. The results of statistical analysis proved that the method is precise, repeatable, reproducible, accurate, and sensitive, and hence can be employed for the quantification of components involved in ABE fermentation. This method was also used to quantify the products and substrates of fermentation samples using individual glucose and a combination of sugars. en_US
dc.description.statementofresponsibility by Manish Kumar, Supreet Sainib and Kalyan Gayenc
dc.format.extent Vol. 6, No. 3, pp. 774-781
dc.language.iso en en_US
dc.publisher Royal Society of Chemistry en_US
dc.subject Abe fermentations en_US
dc.subject Acetone-butanol-ethanol fermentation en_US
dc.subject Clostridium acetobutylicum en_US
dc.subject Column temperature en_US
dc.subject Correlation coefficient en_US
dc.subject Linear relationships en_US
dc.subject Relative standard deviations en_US
dc.subject Simultaneous use en_US
dc.title Acetone-butanol-ethanol fermentation analysis using only high performance liquid chromatography en_US
dc.type Article en_US
dc.relation.journal Analytical Methods

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